International Journal of Cell Cloning, Vol 1, 191-205, Copyright © 1983 by AlphaMed Press
Stem cells from peripheral blood and bone marrow: a comparative evaluation of the hemopoietic potential in the dog
A Raghavachar, O Prummer, TM Fliedner, W Calvo and IB Steinbach
The kinetics and pattern of hemopoietic recovery after supralethal
total-body irradiation (TBI) were compared after transfusion of
cryopreserved autografts derived from peripheral blood and bone marrow.
Fractionated TBI was given in three doses of 6 Gy each at intervals of 48
h. Grafts of peripheral blood mononuclear cells (MNC) were collected by
means of continuous-flow centrifugation and by using the mobilizing agent,
dextran sulphate. Autografts were adjusted to contain equal numbers of
committed progenitor cells (CFU-GM). Dogs grafted with blood- derived MNC
(group A) and with MNC from bone marrow (group B) all received about 1 X
10(5) CFU-GM per kg body weight. In all dogs consistent hemopoietic
engraftment was achieved. Comparing the pattern of regeneration of the
granulocytes, group A dogs showed a significant regeneratory advantage over
group B dogs, particularly during the first 20 days after transplantation.
Lymphoid recovery was more rapid in group A until day 14. In both groups,
blood lymphocytes remained below normal values beyond day 100. The
regeneration patterns of the platelets and reticulocytes revealed no
significant differences. These results are in agreement with the hypothesis
that there are differences in the relationship between CFU-GM content and
hemopoietic potential of autografts from different sources.
