International Journal of Cell Cloning, Vol 1, 343-359, Copyright © 1983 by AlphaMed Press
Incidence and characteristics of colony-forming units fibroblasts (CFU- F) in the bone marrow of weanling mice treated with cytosine arabinoside and phenylhydrazine: correlation with CFU-S, progenitors of diffusion-chamber colonies (CFU-D), and CFU-C
Z Ben-Ishay, A Borenstein, S Sharon and G Prindull
A study of murine adherent marrow cells (AMC) under conditions of high and
low concentrations of hematopoietic stem cells and progenitors was carried
out. In one group of weanling mice, decreased marrow cellularity and
increased concentrations of CFU-S, CFU-D, and CFU-C were observed two days
after administration of two consecutive intraperitoneal (i.p.) cytosine
arabinoside (Ara-C) injections (2 x 200 ng/kg, at 6 h interval). Fibroblast
colony-forming units (CFU-F) of this marrow were studied. In a second group
of mice, which were given three i.p. phenylhydrazine (PHZ) injections (3 x
60 mg/kg on days 0, 1, and 3), CFU-S and CFU-C levels were unchanged or
lowered 6 days after the start of PHZ administration. In these animals,
however, the number of CFU-D was four times higher than in controls. The
study of CFU-F in experiments of groups 1 and 2 indicated a high
concentration of these progenitors in group 1 and lower concentration in
group 2. Furthermore, fibroblastoid colonies produced in vitro by CFU-F of
Ara-C-treated marrow were significantly larger than colonies from control
marrow, and they markedly inhibited G/M colonies in split-phase agar
cultures. By contrast, fibroblastoid colonies produced by PHZ-treated
marrow were of regular size and did not inhibit G/M colonies from test bone
marrow.
