Stem Cells
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International Journal of Cell Cloning, Vol 1, 412-428, Copyright © 1983 by AlphaMed Press

ORIGINAL ARTICLES

Enhancement of human myeloid stem cell growth in vitro

JR Wells, A Sullivan and DW Golde

Human myeloid stem cell growth in agar was enhanced by the addition of human plasma, red-cell lysate, hemin, and beta-mercaptoethanol to the culture system. Using conditioned media from a human T-cell or leukocyte-conditioned media as a source of colony-stimulating activity, the number of colonies was increased due to enhancement by an average of 54%. Analysis of colony types indicated that over 80% of the increase in colony number at eight days was due to granulocytic colony growth. Light density marrow cells cultured with enhancing agents produced colony levels of approximately 12-27 CFU-C/2 x 10(3) cells.




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STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
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Copyright © 1983 by AlphaMed Press.