Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kadouri, A.
Right arrow Articles by Zipori, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kadouri, A.
Right arrow Articles by Zipori, D.

International Journal of Cell Cloning, Vol 10, 299-308, Copyright © 1992 by AlphaMed Press

ORIGINAL ARTICLES

Dynamic changes in cytokine secretion by stromal cells during prolonged maintenance under protein-free conditions

A Kadouri, R Kompier, J Honigwachs-Sha'anani, J Toledo, N Brosh, D Sternberg, A Levy, E Tzehoval and D Zipori
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.

Stromal cells of bone marrow origin produce a variety of known cytokines and some factors exhibiting apparently new biological activities. Several of these were identified by the study of cell to cell interactions and were not found in detectable amounts in media conditioned by the cells. We describe here a culture system that enables the release of stromal cytokines into medium free of any added proteins and supplemented with peptides from casein hydrolysate (0.1%). The absence of serum proteins allows extensive concentration and monitoring of activities that are otherwise undetectable. Stromal cells of the MBA-2.1 clonal cell line were seeded in a stationary bed reactor packed with a carrier of non-woven fabric matrix. After a proliferation phase with serum containing medium, the cells were maintained for over 10 months in protein-free medium. Throughout this extended incubation in the absence of serum or serum replacing proteins, stromal cells retained their viability and continuously released transforming growth factor-beta (TGF-beta), macrophage-colony stimulating factor (M-CSF) and restrictin-P, a cytotoxic factor that specifically arrested the growth of plasmacytoma cells. In addition, interleukin-6 (IL-6) was first undetectable, and later in culture its titer reached a maximum of 180,000 international units (IU)/ml. Concomitantly, the production of restrictin-P diminished and reached its lowest levels at the end of 10 months. The results may imply a possible causal relationship between the expression of IL-6 and restrictin-P, since no similarly significant changes were observed in the titers of M-CSF and TGF-beta. This novel bioreactor system may be adaptable for efficient production of different cytokines under absolute serum-free conditions.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Brosh, D. Sternberg, J. Honigwachs-Sha'anani, B.-C. Lee, Y. Shav-Tal, E. Tzehoval, L. M. Shulman, J. Toledo, Y. Hacham, P. Carmi, et al.
The Plasmacytoma Growth Inhibitor Restrictin-P Is an Antagonist of Interleukin 6 and Interleukin 11
J. Biol. Chem., December 8, 1995; 270(49): 29594 - 29600.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 1992 by AlphaMed Press.