Stem Cells, Vol 13, 273-280, Copyright © 1995 by AlphaMed Press
Characterization of enriched CD34+ cells from healthy volunteers and those from patients treated with chemotherapy plus granulocyte colony- stimulating factor (G-CSF)
T Suzuki, K Muroi, H Tomizuka, Y Amemiya and Y Miura
Department of Medicine, Jichi Medical School, Tochigi-ken, Japan.
CD34+ cells were enriched, using a panning method, from peripheral blood
(PB) and bone marrow (BM) of healthy volunteers and of patients treated
with chemotherapy plus granulocyte colony-stimulating factor (G- CSF). In
healthy volunteers, PB CD34+ cells expressed CD33 and CD13 at a higher
frequency than BM CD34+ cells, and PB CD34+ cells contained a greater
number of burst-forming units-erythroid (BFU-E) than colony- forming units
granulocyte-macrophage (CFU-GM). Administration of G-CSF to healthy
volunteers induced a marked increase in the number of PB CD34+ cells,
although the proportions of those expressing CD33, CD13, and c-kit among
these cells as well as colony-forming ability were not changed before and
after G-CSF administration. There were no significant differences in
surface antigens on PB CD34+ cells between healthy volunteers and patients
after chemotherapy plus G-CSF, except for low expression of c-kit in the PB
of patients. However, PB CD34+ cells from patients contained almost the
same number of CFU-GM as BFU- E. These results indicate that there were
clear differences in the features of CD34+ cells from BM and from PB, and
between healthy volunteers and patients after chemotherapy plus G-CSF.
Enriched CD34+ cells are useful for analyzing the characteristics of
hematopoietic progenitor cells, and such analysis may predict the
usefulness of autologous or allogeneic peripheral blood stem cell
transplantation.
