| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan;
b Department of Environment Pathology, School of Preventive Medicine, Norman Bethune University of Medical Sciences, Changchun, China;
c Department of Scientific Research, Changchun Research Institute of Biological Production, Ministry of Health, Changchun, China
Key Words. Pluripotent hemopoietic stem cells • Intrathymic injection • c-kit
Dr. Susumu Ikehara, First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570, Japan.
To investigate whether hemopoietic stem cells (HSCs) can differentiate into all lineage cells even in the thymus, we injected two types of HSCs (c-kit+ and c-kit<low cells) obtained from C57BL/6 Ly5.1 mice directly into the thymus of 7.5 Gy-irradiated C57BL/6 Ly5.2 mice. When c-kit<low cells (low density/lineage–/CD71–/major histocompatibility complex class Ihigh/Sca-1+/Thy-1low/ c-kit<low) were injected, donor-derived (Ly5.1) cells were detected on day 8 after intrathymic (i.t.) injection, and the number reached a maximum on day 24 after injection. Granulocytes and macrophages were also detected on day 8 after injection. However, B220+ B cells were observed on day 13. Eighteen days after i.t. injection, the injected lobes showed red color due to the synchronous development of erythroid cells. Histological studies revealed the development not only of erythroid lineage cells but also of megakaryocytes in the thymus. In contrast, when c-kit+ cells were injected, a significant number of donor-derived cells were detected on day 5 after i.t. injection (three days earlier than in the case of c-kit<low cell injection). The differentiation into erythroid lineage cells was also observed six days earlier than when c-kit<low HSCs were injected. These findings suggest that c-kit<low HSCs are more primitive than c-kit+ HSCs, although both can differentiate into all lineage cells after i.t. injection.
This article has been cited by other articles:
|
|
 |
|
  S. H.A. Wong, K. N. Lowes, I. Bertoncello, A. F. Quigley, P. J. Simmons, M. J. Cook, A. J. Kornberg, and R. M.I. Kapsa Evaluation of Sca-1 and c-Kit As Selective Markers for Muscle Remodelling by Nonhemopoietic Bone Marrow Cells Stem Cells, June 1, 2007; 25(6): 1364 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Klarmann, M. Ortiz, M. Davies, and J. R. Keller Identification of in vitro growth conditions for c-Kit-negative hematopoietic stem cells Blood, November 1, 2003; 102(9): 3120 - 3128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-X. Lian, T. Okada, X.-S. He, H. Kita, Y.-J. Liu, A. A. Ansari, K. Kikuchi, S. Ikehara, and M. E. Gershwin Heterogeneity of Dendritic Cells in the Mouse Liver: Identification and Characterization of Four Distinct Populations J. Immunol., March 1, 2003; 170(5): 2323 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-X. Lian, T. Okada, H. Kita, T. Hsu, L. D. Shultz, K. Dorshkind, A. A. Ansari, M. Naiki, S. Ikehara, and M. E. Gershwin Age-Related Alterations in the Lymphohematopoietic and B-Lineage Precursor Populations in NZB Mice Stem Cells, July 1, 2002; 20(4): 293 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Yang, H. Hisha, Y. Cui, T. Fan, T. Jin, Q. Li, Z. Lian, N. Hosaka, Y. Li, and S. Ikehara A New Assay Method for Late CFU-S Formation and Long-Term Reconstituting Activity Using a Small Number of Pluripotent Hemopoietic Stem Cells Stem Cells, May 1, 2002; 20(3): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Reid, A. Ritchie, L. Boring, J. Gosling, S. Cooper, G. Hangoc, I. F. Charo, and H. E. Broxmeyer Enhanced Myeloid Progenitor Cell Cycling and Apoptosis in Mice Lacking the Chemokine Receptor, CCR2 Blood, March 1, 1999; 93(5): 1524 - 1533. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
