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Estimation of Extent of Cell Death in Different Stages of Normal Murine Hematopoiesis

Emanuel Neas^a, Ludk efc^a, Karel ulc^a, Edda Barthel^b, Hans-Joachim Seidel^b

^a Institute of Pathophysiology, First Medical Faculty, Charles University, Prague, Czech Republic;
^b Institute of Occupational and Social Medicine, University of Ulm, Federal Republic of Germany

Key Words. Apoptosis • Hematopoiesis • Cell cycle • Hydroxyurea • Mouse • Turnover of blood cells • Kinetics

Prof. Emanuel Neas, Institute of Pathophysiology, First Faculty of Medicine, Charles University, U nemocnice 5, 128 53 Praha 2, Czech Republic.

Murine hematopoiesis has been analyzed by many authors, and available data allow for quantitative evaluation of this dynamic process. In this study, the capacity of several populations of the bone marrow clonogenic cells (progenitors) to produce blood cells was compared with their actual production. The cell cycle progression rate was directly measured in the following types of hematopoietic progenitors: day 8 colony-forming units-spleen, GM-colony-forming cells, BFU-E, and CFU-E in normal mice. The cell cycle progression rates of the individual progenitors, together with their numbers in the whole hematopoietic tissue, were used to calculate the absolute numbers produced daily in each population. The data reviewed from literature were analyzed in parallel. The capacity of the progenitors to produce mature blood cells was derived from the daily production of progenitors multiplied by their clonogenic potential. This theoretical capacity to produce blood cells was compared to the actual blood cell production determined from the turnover of circulating blood elements. The comparison strongly suggested an intensive cell death rate occurring at the early stages of differentiation and its decline as the hematopoietic cells become more differentiated and mature.

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