HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lian, Z. Articles by Ikehara, S. Search for Related Content PubMed PubMed Citation Articles by Lian, Z. Articles by Ikehara, S.

c-kit^<low Pluripotent Hemopoietic Stem Cells Form CFU-S on Day 16

First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan

Key Words. CFU-S • Pluripotent hemopoietic stem cell • c-kit • Mouse

Dr. Susumu Ikehara, First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan.

Using Ly5 congenic mice, we characterized the early differentiation step of pluripotent hemopoietic stem cells. Lineage^– (Lin^–)/CD71^– cells in the bone marrow cells were separated into major histocompatibility complex (MHC) class I^high/c-kit^low and MHC class I^high/c-kit^<low populations from C57BL/6 Ly5.1 male mice. These two populations (1,000 cells) were transplanted into lethally irradiated (5.5 Gy x 2) C57BL/6 Ly5.2 female mice. Colony-forming unit in spleen (CFU-S) assays were carried out on days 10, 12, 14, 16, and 20. In the mice that received c-kit^low cells, CFU-S were first detected on day 12, and the CFU-S counts gradually increased. In contrast, no visible colony was detected until day 14 in the mice that received c-kit^<low cells; CFU-S were first observed on day 16. Donor-derived (Ly5.1⁺) cells, such as B cells, T cells, and myeloid cells, were detected by fluorescence-activated cell sorter analyses, and donor-derived erythroid cells were detected by polymerase chain reaction analyses using Y-chromosome-specific primers. Donor-derived cells in the recipients of c-kit^low cells were detected in the spleen, bone marrow, and peripheral blood on day 12 after transplantation, while they were detected on day 16 in the mice that received c-kit^<low cells. Therefore, c-kit^<low cells have the capacity not only to form CFU-S on day 16 but also to reconstitute the recipients with donor-derived hematolymphoid cells 16 days after transplantation.

This article has been cited by other articles:

				Z.-X. Lian, T. Okada, H. Kita, T. Hsu, L. D. Shultz, K. Dorshkind, A. A. Ansari, M. Naiki, S. Ikehara, and M. E. Gershwin Age-Related Alterations in the Lymphohematopoietic and B-Lineage Precursor Populations in NZB Mice Stem Cells, July 1, 2002; 20(4): 293 - 300. [Abstract] [Full Text] [PDF]

				G. Yang, H. Hisha, Y. Cui, T. Fan, T. Jin, Q. Li, Z. Lian, N. Hosaka, Y. Li, and S. Ikehara A New Assay Method for Late CFU-S Formation and Long-Term Reconstituting Activity Using a Small Number of Pluripotent Hemopoietic Stem Cells Stem Cells, May 1, 2002; 20(3): 241 - 248. [Abstract] [Full Text] [PDF]

				H. Hayashi, J. Toki, L. Zhexiong, K. Sugiura, K. Inoue, and S. Ikehara Long-Term (>1 year) Analyses of Chimerism and Tolerance in Mixed Allogeneic Chimeric Mice Using Normal Mouse Combinations Stem Cells, July 1, 2000; 18(4): 273 - 280. [Abstract] [Full Text]

				S. Ikehara Pluripotent Hemopoietic Stem Cells in Mice and Humans Experimental Biology and Medicine, February 1, 2000; 223(2): 149 - 155. [Abstract] [Full Text]

				B. Feng, M. Inaba, Z. Lian, Y. Cui, J. Toki, T. Ito, T. Jin, T. Fan, G. Yang, C. Yu, et al. Development of Mouse Dendritic Cells from Lineage-Negative c-kit‹low Pluripotent Hemopoietic Stem Cells In Vitro Stem Cells, January 1, 2000; 18(1): 53 - 60. [Abstract] [Full Text]