| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
RAPID COMMUNICATION |
Laboratory of Human Stem Cells, Institute of Experimental Cardiology, National Cardiology Research Center of the Russian Ministry of Health, Moscow, Russia
Key Words. Umbilical cord • Mesenchymal stem cells • Cell culture
Yuri A. Romanov, Ph.D., Laboratory of Human Stem Cells, Institute of Experimental Cardiology, National Cardiology Research Center, 3rd Cherepkovskaya Str. 15-A, Moscow 121552, Russian Federation. Telephone: 7-095-414-6949; Fax: 7-095-149-2652; e-mail: romanov{at}cardio.ru
Mesenchymal stem cells (MSCs) have the capability for renewal and differentiation into various lineages of mesenchymal tissues. These features of MSCs attract a lot of attention from investigators in the context of cell-based therapies of several human diseases. Despite the fact that bone marrow represents the main available source of MSCs, the use of bone marrow-derived cells is not always acceptable due to the high degree of viral infection and the significant drop in cell number and proliferative/differentiation capacity with age. Thus, the search for possible alternative MSC sources remains to be validated. Umbilical cord blood is a rich source of hematopoietic stem/progenitor cells and does not contain mesenchymal progenitors. However, MSCs circulate in the blood of preterm fetuses and may be successfully isolated and expanded. Where these cells home at the end of gestation is not clear. In this investigation, we have made an attempt to isolate MSCs from the subendothelial layer of umbilical cord vein using two standard methodological approaches: the routine isolation of human umbilical vein endothelial cell protocol and culture of isolated cells under conditions appropriate for bone-marrow-derived MSCs. Our results suggest that cord vasculature contains a high number of MSC-like elements forming colonies of fibroblastoid cells that may be successfully expanded in culture. These MSC-like cells contain no endothelium- or leukocyte-specific antigens but express alpha-smooth muscle actin and several mesenchymal cell markers. Therefore, umbilical cord/placenta stroma could be regarded as an alternative source of MSCs for experimental and clinical needs.
This article has been cited by other articles:
|
|
 |
|
  N. Kaltz, A. Funari, S. Hippauf, B. Delorme, D. Noel, M. Riminucci, V. R. Jacobs, T. Haupl, C. Jorgensen, P. Charbord, et al. In Vivo Osteoprogenitor Potency of Human Stromal Cells from Different Tissues Does Not Correlate with Expression of POU5F1 or Its Pseudogenes Stem Cells, September 1, 2008; 26(9): 2419 - 2424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Moon, J. R. Lee, B. C. Jee, C. S. Suh, S. H. Kim, H. J. Lim, and H. K. Kim Successful vitrification of human amnion-derived mesenchymal stem cells Hum. Reprod., August 1, 2008; 23(8): 1760 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.E. Schwab, P. Hutchinson, and C.E. Gargett Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells Hum. Reprod., April 1, 2008; 23(4): 934 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Hess, T. P. Craft, L. Wirthlin, S. Hohm, P. Zhou, W. C. Eades, M. H. Creer, M. S. Sands, and J. A. Nolta Widespread Nonhematopoietic Tissue Distribution by Transplanted Human Progenitor Cells with High Aldehyde Dehydrogenase Activity Stem Cells, March 1, 2008; 26(3): 611 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Troyer and M. L. Weiss Concise Review: Wharton's Jelly-Derived Cells Are a Primitive Stromal Cell Population Stem Cells, March 1, 2008; 26(3): 591 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gandia, A. Arminan, J. M. Garcia-Verdugo, E. Lledo, A. Ruiz, M D. Minana, J. Sanchez-Torrijos, R. Paya, V. Mirabet, F. Carbonell-Uberos, et al. Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction Stem Cells, March 1, 2008; 26(3): 638 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Magatti, S. De Munari, E. Vertua, L. Gibelli, G. S. Wengler, and O. Parolini Human Amnion Mesenchyme Harbors Cells with Allogeneic T-Cell Suppression and Stimulation Capabilities Stem Cells, January 1, 2008; 26(1): 182 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Secco, E. Zucconi, N. M. Vieira, L. L.Q. Fogaca, A. Cerqueira, M. D. F. Carvalho, T. Jazedje, O. K. Okamoto, A. R. Muotri, and M. Zatz Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood! Stem Cells, January 1, 2008; 26(1): 146 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Can and S. Karahuseyinoglu Concise Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem Cells, November 1, 2007; 25(11): 2886 - 2895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Park, K. H. Jung, S. H. Kim, K. S. Kim, M. R. Choi, Y. Kim, and Y. G. Chai Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein Stem Cells, August 1, 2007; 25(8): 2044 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Christopherson, T. Bakhshi, S. Bodie, S. Kidd, R. Zabriskie, and S. Ramin Mesenchymal Stem Cells from the Wharton's Jelly of Umbilical Cord Segments Support the Maintenance of Long Term Culture-Initiating Cells from Cord Blood. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 3650 - 3650. [Abstract] [PDF]
|
|
|
|
 |
|
 K. H. Wu, Y. L. Liu, B. Zhou, and Z. C. Han Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells Eur. J. Cardiothorac. Surg., November 1, 2006; 30(5): 770 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Koerner, D. Nesic, J. D. Romero, W. Brehm, P. Mainil-Varlet, and S. P. Grogan Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells. Stem Cells, June 1, 2006; 24(6): 1613 - 1619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-R. Son, L. A. Marquez-Curtis, M. Kucia, M. Wysoczynski, A. R. Turner, J. Ratajczak, M. Z. Ratajczak, and A. Janowska-Wieczorek Migration of Bone Marrow and Cord Blood Mesenchymal Stem Cells In Vitro Is Regulated by Stromal-Derived Factor-1-CXCR4 and Hepatocyte Growth Factor-c-met Axes and Involves Matrix Metalloproteinases Stem Cells, May 1, 2006; 24(5): 1254 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Pountos, E. Jones, C. Tzioupis, D. McGonagle, and P. V. Giannoudis Growing bone and cartilage: THE ROLE OF MESENCHYMAL STEM CELLS J Bone Joint Surg Br, April 1, 2006; 88-B(4): 421 - 426. [Full Text] [PDF]
|
|
|
|
|
|
M. L. Weiss, S. Medicetty, A. R. Bledsoe, R. S. Rachakatla, M. Choi, S. Merchav, Y. Luo, M. S. Rao, G. Velagaleti, and D. Troyer Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's Disease Stem Cells, March 1, 2006; 24(3): 781 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Minguell and A. Erices Mesenchymal Stem Cells and the Treatment of Cardiac Disease Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Leri, J. Kajstura, and P. Anversa Cardiac Stem Cells and Mechanisms of Myocardial Regeneration Physiol Rev, October 1, 2005; 85(4): 1373 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miki, T. Lehmann, H. Cai, D. B. Stolz, and S. C. Strom Stem Cell Characteristics of Amniotic Epithelial Cells Stem Cells, October 1, 2005; 23(10): 1549 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tondreau, N. Meuleman, A. Delforge, M. Dejeneffe, R. Leroy, M. Massy, C. Mortier, D. Bron, and L. Lagneaux Mesenchymal Stem Cells Derived from CD133-Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity Stem Cells, September 1, 2005; 23(8): 1105 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sarugaser, D. Lickorish, D. Baksh, M. M. Hosseini, and J. E. Davies Human Umbilical Cord Perivascular (HUCPV) Cells: A Source of Mesenchymal Progenitors Stem Cells, February 1, 2005; 23(2): 220 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Yen, H.-I Huang, C.-C. Chien, H.-Y. Jui, B.-S. Ko, M. Yao, C.-T. Shun, M.-l. Yen, M.-C. Lee, and Y.-C. Chen Isolation of Multipotent Cells from Human Term Placenta Stem Cells, January 1, 2005; 23(1): 3 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Panepucci, J. L.C. Siufi, W. A. Silva Jr., R. Proto-Siquiera, L. Neder, M. Orellana, V. Rocha, D. T. Covas, and M. A. Zago Comparison of Gene Expression of Umbilical Cord Vein and Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells, December 1, 2004; 22(7): 1263 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Abe, C. Boyer, X. Liu, F. Q. Wen, T. Kobayashi, Q. Fang, X. Wang, M. Hashimoto, J. G. Sharp, and S. I. Rennard Cells Derived from the Circulation Contribute to the Repair of Lung Injury Am. J. Respir. Crit. Care Med., December 1, 2004; 170(11): 1158 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H T Hassan and M El-Sheemy Adult bone-marrow stem cells and their potential in medicine J R Soc Med, October 1, 2004; 97(10): 465 - 471. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Abedin, Y. Tintut, and L. L. Demer Mesenchymal Stem Cells and the Artery Wall Circ. Res., October 1, 2004; 95(7): 671 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fukuchi, H. Nakajima, D. Sugiyama, I. Hirose, T. Kitamura, and K. Tsuji Human Placenta-Derived Cells Have Mesenchymal Stem/Progenitor Cell Potential Stem Cells, September 1, 2004; 22(5): 649 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kogler, S. Sensken, J. A. Airey, T. Trapp, M. Muschen, N. Feldhahn, S. Liedtke, R. V. Sorg, J. Fischer, C. Rosenbaum, et al. A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential J. Exp. Med., July 19, 2004; 200(2): 123 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Gang, J. A. Jeong, S. H. Hong, S. H. Hwang, S. W. Kim, I. H. Yang, C. Ahn, H. Han, and H. Kim Skeletal Myogenic Differentiation of Mesenchymal Stem Cells Isolated from Human Umbilical Cord Blood Stem Cells, July 1, 2004; 22(4): 617 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bieback, S. Kern, H. Kluter, and H. Eichler Critical Parameters for the Isolation of Mesenchymal Stem Cells from Umbilical Cord Blood Stem Cells, July 1, 2004; 22(4): 625 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Abedin, Y. Tintut, and L. L. Demer Vascular Calcification: Mechanisms and Clinical Ramifications Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1161 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Tsai, J.-L. Lee, Y.-J. Chang, and S.-M. Hwang Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol Hum. Reprod., June 1, 2004; 19(6): 1450 - 1456. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
