HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Selden, C. Articles by Hodgson, H. J.F. Search for Related Content PubMed PubMed Citation Articles by Selden, C. Articles by Hodgson, H. J.F.

Epithelial Colonies Cultured from Human Explanted Liver in Subacute Hepatic Failure Exhibit Hepatocyte, Biliary Epithelial, and Stem Cell Phenotypic Markers

^a Centre for Hepatology, Department of Medicine,
^b Department of Histopathology, and
^c Department of Surgery, Royal Free and University College Medical School, London, United Kingdom

Key Words. Human • Hepatocyte • Stem cell • Biliary epithelial cell • Liver failure

Clare Selden, Ph.D., Centre for Hepatology, Department of Medicine, Royal Free and University College Medical School, Rowland Hill Street, Hampstead, London NW3 2PF, United Kingdom. Telephone: 44-207-433-2854; Fax: 44-207-433-2852; e-mail: c.selden{at}rfc.ucl.ac.uk

The liver in subacute hepatic failure may become enriched for hepatic progenitor cells. Liver tissue from such a patient was collagenase digested and, from the nonparenchymal cell fraction, epithelioid colonies were developed. Albumin and alpha-1-antitrypsin (AAT) were secreted for greater than 120 days from these colonies. Reverse transcription-polymerase chain reaction showed expression of markers of both hepatocyte and biliary epithelial phenotypes (cytokeratins 7, 18, and 19, albumin and AAT, hepatocyte growth factor receptor, transforming growth factor beta receptor type II, gamma-glutamyl transpeptidase, biliary glycoprotein). The cell cycle regulator p21 was also expressed. The POU domain transcription factor octamer-binding protein 4 was present in these cells, but not in RNA or cDNA prepared from adult human liver. These markers were maintained even after 165 days culture. Proliferating epithelial-like cells with combined hepatocyte- and biliary-epithelial-specific functional markers and a stem cell marker can be isolated from the nonparenchymal fraction of liver cells in subacute hepatic failure.

This article has been cited by other articles:

				C. Duret, S. Gerbal-Chaloin, J. Ramos, J.-M. Fabre, E. Jacquet, F. Navarro, P. Blanc, A. Sa-Cunha, P. Maurel, and M. Daujat-Chavanieu Isolation, Characterization, and Differentiation to Hepatocyte-Like Cells of Nonparenchymal Epithelial Cells from Adult Human Liver Stem Cells, July 1, 2007; 25(7): 1779 - 1790. [Abstract] [Full Text] [PDF]

				M. B. Herrera, S. Bruno, S. Buttiglieri, C. Tetta, S. Gatti, M. C. Deregibus, B. Bussolati, and G. Camussi Isolation and Characterization of a Stem Cell Population from Adult Human Liver Stem Cells, December 1, 2006; 24(12): 2840 - 2850. [Abstract] [Full Text] [PDF]

				Y. Y. Dan, K. J. Riehle, C. Lazaro, N. Teoh, J. Haque, J. S. Campbell, and N. Fausto Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages PNAS, June 27, 2006; 103(26): 9912 - 9917. [Abstract] [Full Text] [PDF]