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Stem Cells 2003;21:661-669 www.StemCells.com
© 2003 AlphaMed Press

The Profile of Gene Expression of Human Marrow Mesenchymal Stem Cells

Wilson A. Silva, Jr., Dimas T. Covas, Rodrigo A. Panepucci, Rodrigo Proto-Siqueira, Jorge L.C. Siufi, Dalila L. Zanette, Anemari R.D. Santos, Marco A. Zago

Center for Cell Therapy and Regional Blood Center, Department of Clinical Medicine, Faculty of Medicine, Ribeirão Preto, Brazil

Key Words. Mesenchymal stem cells • SAGE • Gene expression • Hematopoesis

Marco A. Zago, M.D., Ph.D., Hemocentro, R. Tenente Catao, Roxo, 2501, 14051-140 Ribeirao Preto, Brazil. Telephone: 55-16-3963-9361; Fax: 55-16-3963-9309; e-mail: marazago{at}usp.br

Mesenchymal stem cells (MSCs) are multipotent precursors present in adult bone marrow, that differentiate into osteoblasts, adipocytes and myoblasts, and play important roles in hematopoiesis. We examined gene expression of these cells by serial analysis of gene expression, and found that collagen I, secreted protein acidic and rich in cysteine (osteonectin), transforming growth factor beta- (TGF-ß) induced, cofilin, galectin-1, laminin-receptor 1, cyclophilin A, and matrix metalloproteinase-2 are among the most abundantly expressed genes. Comparison with a library of CD34+ cells revealed that MSCs had a larger number of expressed genes in the categories of cell adhesion molecule, extracellular and development. The two types of cells share abundant transcripts of many genes, some of which are highly expressed in myeloid progenitors (thymosin-ß4 and ß10, fos and jun). Interleukin-11 (IL-11), IL-15, IL-27 and IL-10R, IL-13R and IL-17R were the most expressed genes among the cytokines and their receptors in MSCs, and various interactions can be predicted with the CD34+ cells. MSCs express several transcripts for various growth factors and genes suggested to be enriched in stem cells. This study reports the profile of gene expression in MSCs and identifies the important contribution of extracellular protein products, adhesion molecules, cell motility, TGF-ß signaling, growth factor receptors, DNA repair, protein folding, and ubiquination as part of their transcriptome.




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