Stem Cells http://www.stemcellsportal.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, G.-R.
Right arrow Articles by Lau, C.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, G.-R.
Right arrow Articles by Lau, C.-P.

Stem Cells 2005;23:371-382 www.StemCells.com
© 2005 AlphaMed Press

Characterization of Ionic Currents in Human Mesenchymal Stem Cells from Bone Marrow

Gui-Rong Li, Haiying Sun, Xiuling Deng, Chu-Pak Lau

Department of Medicine, Research Centre on Heart, Brain, Hormones and Healthy Aging, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China

Key Words. Ca2+-activated K+ current • Heag K+ current • Human mesenchymal stem cells • Ion channels • Transient outward K+ current • TTX-sensitive Na+ current

Correspondence: Gui-Rong Li, L8–01, Laboratory Block, Faculty of Medicine Building, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Telephone: 852-2819-2830; Fax: 852-2816-2095; e-mail: grli{at}hkucc.hku.hk

This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca2+-activated K+ channel (IKCa) blocker iberiotoxin, a transient outward K+ current (Ito) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K+ current (IKDR)-like ether-à-go-go (eag) K+ channel. In addition, tetrodotoxin-sensitive sodium current (INa.TTX) and nifedipine-sensitive L-type Ca2+ current (ICa.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for IKCa, Kv4.2 and Kv1.4 for Ito, heag1 for IKDR, hNE-Na for INa.TTX, and CACNAIC for ICa.L. These results demonstrate that multiple functional ion channel currents—that is, IKCa, Ito, heag1, INa.TTX, and ICa.L—are expressed in hMSCs from bone marrow.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Tao, C.-P. Lau, H.-F. Tse, and G.-R. Li
Regulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1409 - C1416.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
R. A. Rose, H. Jiang, X. Wang, S. Helke, J. N. Tsoporis, N. Gong, S. C.J. Keating, T. G. Parker, P. H. Backx, and A. Keating
Bone Marrow-Derived Mesenchymal Stromal Cells Express Cardiac-Specific Markers, Retain the Stromal Phenotype, and Do Not Become Functional Cardiomyocytes In Vitro
Stem Cells, November 1, 2008; 26(11): 2884 - 2892.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
X. Bai, J. Ma, Z. Pan, Y.-H. Song, S. Freyberg, Y. Yan, D. Vykoukal, and E. Alt
Electrophysiological properties of human adipose tissue-derived stem cells
Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1539 - C1550.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Tao, C.-P. Lau, H.-F. Tse, and G.-R. Li
Functional ion channels in mouse bone marrow mesenchymal stem cells
Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1561 - C1567.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
K. S. Park, K. H. Jung, S. H. Kim, K. S. Kim, M. R. Choi, Y. Kim, and Y. G. Chai
Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein
Stem Cells, August 1, 2007; 25(8): 2044 - 2052.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
E. Coppi, A. M. Pugliese, S. Urbani, A. Melani, E. Cerbai, B. Mazzanti, A. Bosi, R. Saccardi, and F. Pedata
ATP Modulates Cell Proliferation and Elicits Two Different Electrophysiological Responses in Human Mesenchymal Stem Cells
Stem Cells, July 1, 2007; 25(7): 1840 - 1849.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
P. A. Schweizer, U. Krause, R. Becker, A. Seckinger, A. Bauer, C. Hardt, V. Eckstein, A. D. Ho, M. Koenen, H. A. Katus, et al.
Atrial-Radiofrequency Catheter Ablation Mediated Targeting of Mesenchymal Stromal Cells
Stem Cells, June 1, 2007; 25(6): 1546 - 1551.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. A. Pijnappels, M. J. Schalij, J. van Tuyn, D. L. Ypey, A. A.F. de Vries, E. E. van der Wall, A. van der Laarse, and D. E. Atsma
Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling
Cardiovasc Res, November 1, 2006; 72(2): 282 - 291.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G.-R. Li, X.-L. Deng, H. Sun, S. S.M. Chung, H.-F. Tse, and C.-P. Lau
Ion Channels in Mesenchymal Stem Cells from Rat Bone Marrow
Stem Cells, June 1, 2006; 24(6): 1519 - 1528.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2005 by AlphaMed Press.