Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Stem Cells Vol. 23 No. 6 June 2005, pp. 791 -804
doi:10.1634/stemcells.2004-0232; www.StemCells.com
© 2005 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kania, G.
Right arrow Articles by Wobus, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kania, G.
Right arrow Articles by Wobus, A. M.

Somatic Stem Cell Marker Prominin-1/CD133 Is Expressed in Embryonic Stem Cell–Derived Progenitors

Gabriela Kaniaa, Denis Corbeilb,c, Joerg Fuchsa, Kirill V. Tarasovd, Przemyslaw Blyszczuka, Wieland B. Huttnerc, Kenneth R. Bohelerd, Anna M. Wobusa

a Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany;
b Medical Clinic and Polyclinic I, Carl Gustav Carus University, Dresden, Germany;
c Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany;
d Laboratory of Cardiovascular Science, National Institute on Aging, NIH, Baltimore, Maryland, USA

Key Words. Mouse • Embryonic stem cells • Embryonic carcinoma cells • Blastocysts • Prominin-1 • Nestin • Nanog • Neuronal • Differentiation

Correspondence: Anna M. Wobus, Ph.D., In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany. Telephone: 49-39482-5256; Fax: 49-39482-5481; e-mail: wobusam{at}ipk-gatersleben.de

Prominin-1/CD133 is a plasma membrane marker found in several types of somatic stem cells, including hematopoietic and neural stem cells. To study its role during development and with differentiation, we analyzed its temporal and spatial expression (mRNA and protein) in preimplantation embryos, undifferentiated mouse embryonic stem (ES) cells, and differentiated ES cell progeny. In early embryos, prominin-1 was expressed in trophoblast but not in cells of the inner cell mass; however, prominin-1 transcripts were detected in undifferentiated ES cells. Both ES-derived cells committed to differentiation and early progenitor cells coexpressed prominin-1 with early lineage markers, including the cytoskeletal markers (nestin, cytokeratin 18, desmin), fibulin-1, and valosin-containing protein. After spontaneous differentiation at terminal stages, prominin-1 expression was downregulated and no coexpression with markers characteristic for neuroectodermal, mesodermal, and endodermal cells was found. Upon induction of neuronal differentiation, some prominin-1–positive cells, which coexpressed nestin and showed the typical morphology of neural progenitor cells, persisted until terminal stages of differentiation. However, no coexpression of prominin-1 with markers of differentiated neural cells was detected. In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
D. J. Vander Griend, W. L. Karthaus, S. Dalrymple, A. Meeker, A. M. DeMarzo, and J. T. Isaacs
The Role of CD133 in Normal Human Prostate Stem Cells and Malignant Cancer-Initiating Cells
Cancer Res., December 1, 2008; 68(23): 9703 - 9711.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
Y. Hori, M. Fukumoto, and Y. Kuroda
Enrichment of Putative Pancreatic Progenitor Cells from Mice by Sorting for Prominin1 (CD133) and Platelet-Derived Growth Factor Receptor {beta}
Stem Cells, November 1, 2008; 26(11): 2912 - 2920.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G. Kania, P. Blyszczuk, A. Valaperti, T. Dieterle, B. Leimenstoll, S. Dirnhofer, H. Zulewski, and U. Eriksson
Prominin-1+/CD133+ bone marrow-derived heart-resident cells suppress experimental autoimmune myocarditis
Cardiovasc Res, November 1, 2008; 80(2): 236 - 245.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Coskun, H. Wu, B. Blanchi, S. Tsao, K. Kim, J. Zhao, J. C. Biancotti, L. Hutnick, R. C. Krueger Jr., G. Fan, et al.
From the Cover: CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain
PNAS, January 22, 2008; 105(3): 1026 - 1031.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. Yoshida, M. Amanai, T. Fukui, E. Kajikawa, M. Brahmajosyula, A. Iwahori, Y. Nakano, S. Shoji, J. Diebold, H. Hessel, et al.
Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in mice
Development, November 1, 2007; 134(21): 3941 - 3952.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Lagares, H.-Y. Li, X.-F. Zhou, and C. Avendano
Primary Sensory Neuron Addition in the Adult Rat Trigeminal Ganglion: Evidence for Neural Crest Glio-Neuronal Precursor Maturation
J. Neurosci., July 25, 2007; 27(30): 7939 - 7953.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Lee, D. Medina, A. Tsimelzon, S. K. Mohsin, S. Mao, Y. Wu, and D. C. Allred
Alterations of Gene Expression in the Development of Early Hyperplastic Precursors of Breast Cancer
Am. J. Pathol., July 1, 2007; 171(1): 252 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Gu, J. Yuan, M. Wills, and S. Kasper
Prostate Cancer Cells with Stem Cell Characteristics Reconstitute the Original Human Tumor In vivo
Cancer Res., May 15, 2007; 67(10): 4807 - 4815.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Nakano, K. Satoh, Y. Fukumoto, Y. Ito, Y. Kagaya, N. Ishii, K. Sugamura, and H. Shimokawa
Important Role of Erythropoietin Receptor to Promote VEGF Expression and Angiogenesis in Peripheral Ischemia in Mice
Circ. Res., March 16, 2007; 100(5): 662 - 669.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E.A. Campbell, L. O'Hara, R.D. Catalano, A.M. Sharkey, T.C. Freeman, and M. H. Johnson
Temporal expression profiling of the uterine luminal epithelium of the pseudo-pregnant mouse suggests receptivity to the fertilized egg is associated with complex transcriptional changes
Hum. Reprod., October 1, 2006; 21(10): 2495 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
C. Wiese, A. Rolletschek, G. Kania, A. Navarrete-Santos, S. V. Anisimov, B. Steinfarz, K. V. Tarasov, S. A. Brugh, I. Zahanich, C. Ruschenschmidt, et al.
Signals from Embryonic Fibroblasts Induce Adult Intestinal Epithelial Cells to Form Nestin-Positive Cells with Proliferation and Multilineage Differentiation Capacity In Vitro
Stem Cells, September 1, 2006; 24(9): 2085 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. P. Raikwar, T. Mueller, and N. Zavazava
Strategies for Developing Therapeutic Application of Human Embryonic Stem Cells
Physiology, February 1, 2006; 21(1): 19 - 28.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
F. Ravandi and Z. Estrov
Eradication of Leukemia Stem Cells as a New Goal of Therapy in Leukemia
Clin. Cancer Res., January 15, 2006; 12(2): 340 - 344.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.stemcellsportal.com/
Copyright © 2005 by AlphaMed Press.