HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2005-0073v1 23/9/1276    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marshall, G. P. Articles by Steindler, D. A. Search for Related Content PubMed PubMed Citation Articles by Marshall, G. P., II Articles by Steindler, D. A.

Ionizing Radiation Enhances the Engraftment of Transplanted In Vitro–Derived Multipotent Astrocytic Stem Cells

Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Gainesville, Florida, USA

Key Words. Green fluorescent protein • Irradiation • Multipotent astrocytic stem cell • Neurosphere • Neuroblast • Neural stem cell • Subependymal zone

Correspondence: Gregory P. Marshall II, Ph.D., 1600 SW Archer Road, Gainesville, Florida 32611, USA. Telephone: 352-392-0231; Fax: 352-392-0025; e-mail: gpm2{at}ufl.edu

The subependymal zone (SEZ) is a region of persistent neurogenesis in the adult mammalian brain containing a neural stem cell (NSC) pool that continuously generates migratory neuroblasts that travel in chains through the rostral migratory stream (RMS) to the olfactory bulb (OB), where they differentiate and functionally integrate into existing neural circuitry. NSCs can be isolated from the SEZ and cultured to generate either neurospheres (NSs) or multipotent astrocytic stem cells (MASCs), with both possessing the stem cell characteristics of multipotency and self-renewal. NSs and MASCs home to the SEZ after transplantation into the lateral ventricle (LV) and contribute to neuroblast migration, with minimal engraftment into the OB observed in the adult mouse. Recent studies have compared the relatively uncharacterized NSC with the more established hematopoietic stem cell (HSC) in an effort to determine the level of stemness possessed by the NSC. Depletion of native HSCs in the bone marrow by lethal irradiation (LI) is necessary to maximize functional engraftment of donor HSCs. Our data show that the NSC pool and neuroblasts in the SEZ can be significantly and permanently depleted by exposure to LI. Attenuation of donor-derived migratory neuroblast engraftment into the OB is observed after transplantation of gfp⁺ MASCs into the LV of LI animals, whereas engraftment is significantly enhanced after transplantation into animals exposed to sublethal levels of ionizing radiation. By increasing receptiveness of the NSC niche through depletion of indigenous cells, the adult SEZ-RMS-OB can be used as a model to further characterize the NSC.

This article has been cited by other articles:

				G. P. Marshall II, E. D. Laywell, T. Zheng, D. A. Steindler, and E. W. Scott In Vitro-Derived "Neural Stem Cells" Function as Neural Progenitors Without the Capacity for Self-Renewal Stem Cells, March 1, 2006; 24(3): 731 - 738. [Abstract] [Full Text] [PDF]