Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Stem Cells Vol. 23 No. 9 October 2005, pp. 1324 -1332
doi:10.1634/stemcells.2005-0085; www.StemCells.com
© 2005 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, Y.
Right arrow Articles by Broxmeyer, H. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, Y.
Right arrow Articles by Broxmeyer, H. E.

SDF-1/CXCL12 Enhances Survival and Chemotaxis of Murine Embryonic Stem Cells and Production of Primitive and Definitive Hematopoietic Progenitor Cells

Ying Guoa,b, Giao Hangoca,b, Huimin Biana,b, Louis M. Pelusa,b, Hal E. Broxmeyera,b

a Department of Microbiology/Immunology, The Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana, USA;
b Walther Cancer Institute, Indianapolis, Indiana, USA

Key Words. Embryonic stem cells • SDF-1/CXCL12 • Apoptosis • Chemotaxis • Differentiation

Correspondence: Hal E. Broxmeyer, Ph.D., Walther Oncology Center, Indiana University School of Medicine, 1044 West Walnut Street, R4-302, Indianapolis, Indiana 46202, USA. Telephone: 317-274-7510; Fax: 317-274-7592; e-mail: hbroxmey{at}iupui.edu

Understanding embryonic stem cell (ESC) regulation is important for realizing how best to control their growth and differentiation ex vivo for potential therapeutic benefit. Stromal cell–derived factor-1 (SDF-1/CXCL12) and its receptor, CXCR4, have been implicated as important regulators of a number of fetal and adult cell functions, including survival/antiapoptosis and migration/homing of hematopoietic stem and progenitor cells. We hypothesized that the SDF-1/CXCL12–CXCR4 axis would also be important for regulation of murine ESC functions. ESCs secreted low levels of SDF-1/CXCL12 and expressed low levels of CXCR4; however, both increased with differentiation of ESCs. Endogenously produced/released SDF-1/CXCL12 enhanced survival/antiapoptosis of ESCs in the presence of leukemia inhibitory factor but absence of serum, and survival/antiapoptosis was further enhanced by exogenous administration of SDF-1/CXCL12. Furthermore, SDF-1/CXCL12 induced chemotaxis of ESCs, and chemotaxis could be enhanced by diprotin A inhibition of CD26/dipeptidylpeptidase IV. Endogenous and exogenous SDF-1/CXCL12 enhanced embryoid body production of primitive and definitive erythroid, granulocyte-macrophage, and multipotential progenitors. SDF-1/CXCL12 did not noticeably affect production of hemangioblasts. These results demonstrate functional activities of SDF-1/CXCL12 on survival, chemotaxis, and hematopoietic differentiation of murine ESCs that may be relevant for their ex vivo manipulation.




This article has been cited by other articles:


Home page
 JPEN J Parenter Enteral NutrHome page
J. L. Hermsen, F. E. Gomez, Y. Maeshima, Y. Sano, W. Kang, and K. A. Kudsk
Decreased Enteral Stimulation Alters Mucosal Immune Chemokines
JPEN J Parenter Enteral Nutr, January 1, 2008; 32(1): 36 - 44.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. Palumbo, B. G. Galvez, T. Pusterla, F. De Marchis, G. Cossu, K. B. Marcu, and M. E. Bianchi
Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-{kappa}B activation
J. Cell Biol., October 8, 2007; 179(1): 33 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Xu, A. Mora, H. Shim, A. Stecenko, K. L. Brigham, and M. Rojas
Role of the SDF-1/CXCR4 Axis in the Pathogenesis of Lung Injury and Fibrosis
Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 291 - 299.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
M. Shi, J. Li, L. Liao, B. Chen, B. Li, L. Chen, H. Jia, and R. C. Zhao
Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice
Haematologica, July 1, 2007; 92(7): 897 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. Tan, H. Shao, D. Eton, Z. Yang, L. Alonso-Diaz, H. Zhang, A. Schulick, A. S. Livingstone, and H. Yu
Stromal cell-derived factor-1 enhances pro-angiogenic effect of granulocyte-colony stimulating factor
Cardiovasc Res, March 1, 2007; 73(4): 823 - 832.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Huang, Y. Fu, R. A. Charbeneau, T. L. Saunders, D. K. Taylor, K. D. Hankenson, M. W. Russell, L. G. D'Alecy, and R. R. Neubig
Pleiotropic Phenotype of a Genomic Knock-In of an RGS-Insensitive G184S Gnai2 Allele.
Mol. Cell. Biol., September 1, 2006; 26(18): 6870 - 6879.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
Y. Guo, B. Graham-Evans, and H. E. Broxmeyer
Murine Embryonic Stem Cells Secrete Cytokines/Growth Modulators That Enhance Cell Survival/Anti-Apoptosis and Stimulate Colony Formation of Murine Hematopoietic Progenitor Cells
Stem Cells, April 1, 2006; 24(4): 850 - 856.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.stemcellsportal.com/
Copyright © 2005 by AlphaMed Press.