Stem Cells
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First published online October 27, 2005
Stem Cells Vol. 24 No. 1 January 2006, pp. 145 -150
doi:10.1634/stemcells.2005-0432; www.StemCells.com
© 2006 AlphaMed Press

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EMBRYONIC STEM CELLS-CHARACTERIZATION SERIES: COMMENTARY

Establishing Standards for the Characterization of Human Embryonic Stem Cell Lines

Jeanne F. Loringa, Mahendra S. Raob

a Stem Cell Center, Burnham Institute for Medical Research, La Jolla, California, USA;
b Gerontology Research Center, Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, and Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA

Key Words. Human embryonic stem cell • Karyotype • Characterization • Mitochondrial sequencing • Methylation

Correspondence: Mahendra S. Rao, M.D., Ph.D., Invitrogen Corporation, 1600 Faraday Road, Carlsbad, CA 92008, USA. Telephone: 240-344-1781; Fax: 410-558-8249; e-mail: mahendra.rao{at}invitrogen.com. Jeanne F. Loring, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. Telephone: 858-646-3100; Fax: 858-713-6273; e-mail: jloring{at}burnham.org.

As of August 2005, 22 human embryonic stem cell (hESC) lines listed on the National Institutes of Health (NIH) hESC Registry were being distributed to investigators. At a June 2005 meeting of NIH-supported hESC researchers, we proposed that a set of shared standards should be available in order to characterize the cells unambiguously in multiple laboratories. Here, we elaborate such a plan to identify a set of standard methods and to initiate collaborative efforts to validate the standards. The standard assays we propose should be comprehensive enough to ensure that hESC banks can provide a consistent and reliable product for NIH researchers, and inexpensive enough that individual laboratories can afford to use at least some of the methods routinely in their laboratories. We expect that as data accumulate and standards evolve, a core set of tests will become the norm for routine assessment of hESC cultures and that these tests will lay the groundwork for clinical applications of these cells.




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