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TRANSLATIONAL AND CLINICAL RESEARCH |
aStem Cell Transplant Unit; "Aghia Sophia" Children's Hospital, Thivon and Levadias;
bDepartment of Cardiology, Onassis Cardiac Surgery Center;
cDepartment of Nuclear Medicine, Onassis Cardiac Surgery Center;
dImmunology Department and National Histocompatibility Center; "G. Genimmatas" General District Hospital, Athens, Greece
Key Words. Homing • Selected bone marrow-progenitors • Intracoronary infusion • Chronic cardiomyopathy
Correspondence: Evgenios Goussetis, MD, BMT-Unit, "Aghia Sophia" Children's Hospital, Thivon and Levadias, 11527 Athens, Greece. Telephone: (30)-210-7467303; Fax: (30)-210-7778822; e-mail: bmtlab{at}paidon-agiasofia.gr
Received November 25, 2005;
accepted for publication June 15, 2006.
First published online in STEM CELLS EXPRESS June 22, 2006.
Central issues in intracoronary infusion (ICI) of bone marrow (BM)-cells to damaged myocardium for improving cardiac function are the cell number that is feasible and safe to be administrated as well as the retention of cells in the target area. Our study addressed these issues in eight patients with chronic ischemic cardiomyopathy undergoing ICI of selected BM-progenitors. We could immunomagnetically isolate 0.8 ± 0.32 x 107 CD133+ cells and 0.75 ± 0.24 x 107 CD133–CD34+ cells from 310 ± 40 ml BM. After labeling these cells with 99mTc-hexamethylpropylenamineoxime, they were infused into the infarct-related artery without any complication. Scintigraphic images 1 (eight patients) and 24 hours (four patients) after ICI revealed an uptake of 9.2% ± 3.6 and 6.8% ± 2.4 of the total infused radioactivity in the infarcted area of the heart, respectively; the remaining activity was distributed mainly to liver and spleen. We conclude that through ICI of CD133+ and CD133–CD34+ BM-progenitors a significant number of them are preferentially attracted to and retained in the chronic ischemic myocardium.
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