HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2005-0068v1 24/2/399    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takada, K. Articles by Ikehara, S. Search for Related Content PubMed PubMed Citation Articles by Takada, K. Articles by Ikehara, S.

TRANSLATIONAL AND CLINICAL RESEARCH

Treatment of Senile Osteoporosis in SAMP6 Mice by Intra–Bone Marrow Injection of Allogeneic Bone Marrow Cells

First Department of Pathology, Department of Orthopedic Surgery, Department of Obstetrics and Gynecology, Transplantation Center, Kansai Medical University, Moriguchi City, Osaka, Japan

Key Words. Senescence-accelerated mouse prone 6 mice • Osteoporosis • Intra–bone marrow • Bone marrow transplantation • Bone mineral density • Deoxypyridinoline

Correspondence: Susumu Ikehara, M.D., Ph.D., First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan. Telephone: 81-6-6993-9429; Fax: 81-6-6994-8283; e-mail: ikehara{at}takii.kmu.ac.jp

A substrain of the senescence-accelerated mouse, SAMP6 (senescence-accelerated mouse prone 6), spontaneously develops osteoporosis early in life. Therefore, this strain is a useful animal model for developing new strategies for the treatment of osteoporosis in humans. We succeeded in treating osteoporosis in SAMP6 mice after the onset of this disease, using a newly developed method of bone marrow transplantation (BMT): Allogeneic bone marrow cells obtained from normal mouse strains were directly injected into the bone marrow cavity of irradiated SAMP6 mice (intra–bone marrow BMT [IBM-BMT]). After the treatment with IBM-BMT, hematolymphoid cells were completely reconstituted by donor-derived cells, and bone marrow stromal cells were also found to be of donor origin. The treated SAMP6 mice showed histologically-normal trabecular bone. In addition, bone mineral density and urinary deoxypiridinoline, a hallmark of bone destruction, were normalized. When the message levels of cytokines (tumor necrosis factor , interleukin-6 [IL-6], IL-11, and receptor activator of nuclear factor– B ligand [RANKL]) were examined, IL-11, RANKL (from bone marrow stromal cells), and IL-6 (from osteoclasts), which regulate bone remodeling, were restored to levels similar to those in normal B6 mice. These findings indicate that not only the hemopoietic system but also the bone marrow microenvironment were normalized after IBM-BMT, resulting in an amelioration of the imbalance between bone absorption and formation.

This article has been cited by other articles:

				M. Shi, Y. Adachi, A. Shigematsu, N. Koike-Kiriyama, W. Feng, S. Yanai, C. Yunze, Z.-X. Lian, J. Li, and S. Ikehara Intra-Bone Marrow Injection of Donor Bone Marrow Cells Suspended in Collagen Gel Retains Injected Cells in Bone Marrow, Resulting in Rapid Hemopoietic Recovery in Mice Stem Cells, September 1, 2008; 26(9): 2211 - 2216. [Abstract] [Full Text] [PDF]

				M. Dominici, R. Marino, V. Rasini, C. Spano, P. Paolucci, P. Conte, T. J. Hofmann, and E. M. Horwitz Donor cell-derived osteopoiesis originates from a self-renewing stem cell with a limited regenerative contribution after transplantation Blood, April 15, 2008; 111(8): 4386 - 4391. [Abstract] [Full Text] [PDF]

				M. Inaba, Y. Adachi, H. Hisha, N. Hosaka, M. Maki, Y. Ueda, Y. Koike, T. Miyake, J. Fukui, Y. Cui, et al. Extensive Studies on Perfusion Method Plus Intra-Bone Marrow-Bone Marrow Transplantation Using Cynomolgus Monkeys Stem Cells, August 1, 2007; 25(8): 2098 - 2103. [Abstract] [Full Text] [PDF]

				Y. Ueda, M. Inaba, K. Takada, J. Fukui, Y. Sakaguchi, M. Tsuda, M. Omae, T. Kushida, H. Iida, and S. Ikehara Induction of Senile Osteoporosis in Normal Mice by Intra-Bone Marrow-Bone Marrow Transplantation from Osteoporosis-Prone Mice Stem Cells, June 1, 2007; 25(6): 1356 - 1363. [Abstract] [Full Text] [PDF]

				J. Fukui, M. Inaba, Y. Ueda, T. Miyake, N. Hosaka, A-H. Kwon, Y. Sakaguchi, M. Tsuda, M. Omae, Y. Kamiyama, et al. Prevention of Graft-Versus-Host Disease by Intra-Bone Marrow Injection of Donor T Cells Stem Cells, June 1, 2007; 25(6): 1595 - 1601. [Abstract] [Full Text] [PDF]

				Q. Li, H. Hisha, R. Yasumizu, T.-X. Fan, G.-X. Yang, Q. Li, Y.-Z. Cui, X.-L. Wang, C.-Y. Song, S. Okazaki, et al. Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes Stem Cells, May 1, 2007; 25(5): 1186 - 1194. [Abstract] [Full Text] [PDF]

				G. Duque As a Matter of Fat: New Perspectives on the Understanding of Age-Related Bone Loss IBMS BoneKEy, April 1, 2007; 4(4): 129 - 140. [Abstract] [Full Text] [PDF]