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EMBRYONIC STEM CELLS

Differentiation of Human Embryonic Stem Cells into Bipotent Mesenchymal Stem Cells

^aEinstein Center for Human Embryonic Stem Cell Research, Department of Medicine, Division of Hematology and Department of Cell Biology,
^bDepartment of Medicine, Division of Hematology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA

Key Words. Differentiation • Adipocytes • Osteocytes • Mesenchymal stem cells • Human embryonic stem cells

Correspondence: Eric Bouhassira, Ph.D., Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. Telephone: 718-430-2188; Fax: 718-824-3153; e-mail: bouhassi{at}aecom.yu.edu

Received December 23, 2005; accepted for publication April 17, 2006.
First published online in STEM CELLS EXPRESS   April 27, 2006.



Mesenchymal stem cells (MSCs) are multipotent progenitors that can be found in many connective tissues, including fat, bone, cartilage, and muscle. We report here a method to reproducibly differentiate human embryonic stem cells (hESCs) into MSCs that does not require the use of any feeder layer. The cells obtained with this procedure are morphologically similar to bone marrow MSCs, are contact-inhibited, can be grown in culture for about 20 to 25 passages, have an immunophenotype similar to bone marrow MSCs (negative for CD34 and CD45 and positive for CD13, CD44, CD71, CD73, CD105, CD166, human leukocyte antigen [HLA]-ABC, and stage-specific embryonic antigen [SSEA]-4), can differentiate into osteocytes and adipocytes, and can be used as feeder cells to support the growth of undifferentiated hESCs. The ability to produce MSCs from hESCs should prove useful to produce large amounts of genetically identical and genetically modifiable MSCs that can be used to study the biology of MSCs and for therapeutic applications.

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