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TISSUE-SPECIFIC STEM CELLS

N-Cadherin Is Expressed by Putative Stem/Progenitor Cells and Melanocytes in the Human Limbal Epithelial Stem Cell Niche

^aDepartment of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan;
^bInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan;
^cDepartment of Ophthalmology, Osaka University Medical School, Suita, Japan

Key Words. Stem cell • Corneal epithelium • Limbal epithelium • N-cadherin • Melanocyte

Correspondence: Kohji Nishida, M.D., Ph.D., Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan. Telephone: 81-22-717-7294; Fax: 81-22-717-7298; e-mail: knishida{at}oph.med.tohoku.ac.jp

Received March 22, 2006; accepted for publication September 20, 2006.
First published online in STEM CELLS EXPRESS   September 28, 2006.



Corneal epithelial stem cells are known to be localized to the basal layer of the limbal epithelium, providing a model system for epithelial stem cell biology; however, the mechanisms regarding the maintenance of these stem cells in their specialized niche remain poorly understood. N-cadherin is a member of the classic cadherin family and has previously been demonstrated to be expressed by hematopoietic stem cells. In the present study, we demonstrate that N-cadherin is expressed by putative stem/progenitor cells, as well as melanocytes, in the human limbal epithelial stem cell niche. In addition, we demonstrate that upon in vitro culture using 3T3 feeder layers, loss of N-cadherin expression occurs with cell proliferation. These results indicate that N-cadherin may be a critical cell-to-cell adhesion molecule between corneal epithelial stem/progenitor cells and their corresponding niche cells in the limbal epithelium.

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