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First published online November 16, 2006
Stem Cells Vol. 25 No. 3 March 2007, pp. 621 -627
doi:10.1634/stemcells.2006-0390; www.StemCells.com
© 2007 AlphaMed Press

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TISSUE-SPECIFIC STEM CELLS

Differentiation of Adult Mouse Olfactory Precursor Cells into Hair Cells In Vitro

Kharen L. Doylea,b, Anita Kazdaa, Yvonne Horta, Sarah M. McKaya, Sharon Oleskevicha,b

aNeuroscience Research Program, Garvan Institute of Medical Research, Sydney, Australia;
bUniversity of New South Wales, Sydney, Australia

Key Words. Cochlea • Stem cells • Hair cells • Differentiation

Correspondence: Sharon Oleskevich, Ph.D., Neuroscience Research Program, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, Sydney, 2010, New South Wales, Australia. Telephone: 612-9295-8290; Fax: 612-9295-8281; e-mail: s.oleskevich{at}garvan.org.au

Received June 26, 2006; accepted for publication November 9, 2006.
First published online in STEM CELLS EXPRESS   November 16, 2006.



Many forms of deafness result from degeneration of the sensory cells for hearing, the hair cells in the cochlea. Stem cells offer a potential cell-based therapy for the treatment of deafness. Here, we investigate whether adult olfactory precursor cells can differentiate into hair cells in culture. Precursor cells were isolated from mouse olfactory neuroepithelium, were sphere-forming, showed proliferative capacity, and contained cells expressing neuronal and non-neuronal proteins. To induce differentiation, precursor cells were cocultured with cochlear cells and/or cochlear supernatant. Differentiated precursor cells were immunopositive for specific hair cell markers, including myosin VIIa, FM1-43, calretinin, phalloidin, and espin, and resembled hair cells anatomically and immunocytochemically in culture. The results demonstrate for the first time that adult olfactory precursor cells can differentiate into hair cell-like cells, thus providing a potential autotransplantation therapy for hearing loss.




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