Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 11, 2007
Stem Cells Vol. 25 No. 5 May 2007, pp. 1231 -1240
doi:10.1634/stemcells.2006-0617; www.StemCells.com
© 2007 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2006-0617v1
25/5/1231    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maisel, M.
Right arrow Articles by Storch, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maisel, M.
Right arrow Articles by Storch, A.

TISSUE-SPECIFIC STEM CELLS

Transcription Profiling of Adult and Fetal Human Neuroprogenitors Identifies Divergent Paths to Maintain the Neuroprogenitor Cell State

Martina Maisela, Alexander Herrb, Javorina Milosevicc, Andreas Hermanna, Hans-Jörg Habischd, Sigrid Schwarzc, Matthias Kirsche, Gregor Antoniadisf, Rolf Brennerg, Susanne Hallmeyer-Elgnera, Holger Lerched, Johannes Schwarzc, Alexander Storcha

Departments of aNeurology,
bClinical Genetics, and
eNeurosurgery, Technical University of Dresden, Dresden, Germany;
cDepartment of Neurology, University of Leipzig, Leipzig, Germany;
Departments of dNeurology,
fNeurosurgery, and
gDivision for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopaedics, University of Ulm, Ulm, Germany

Key Words. Gene expression profile • Neural stem cells • Neuroprogenitors • Mesenchymal stem cells • Gene chips

Correspondence: Alexander Storch, M.D., Technical University of Dresden, Department of Neurology, Fetscherstrasse 74, 01307 Dresden, Germany, Telephone: 49-351-458-2532; Fax: 49-351-458-4352; e-mail: alexander.storch{at}neuro.med.tu-dresden.de

Received September 29, 2006; accepted for publication January 4, 2007.
First published online in STEM CELLS EXPRESS   January 11, 2007.



Global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs compared with adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern that is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, whereas fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. We present for the first time a detailed transcriptome analysis of human adult NPCs suggesting a relationship between hippocampal NPCs and white matter-derived precursor cells. We further provide a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues.

Disclosure of potential conflicts of interest is found at the end of this article.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS

Copyright © 2007 by AlphaMed Press.