HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2007-0128v1 2007-0128v2 2007-0128v3 25/9/2245    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Aliotta, J. M. Articles by Quesenberry, P. J. Search for Related Content PubMed PubMed Citation Articles by Aliotta, J. M. Articles by Quesenberry, P. J.

STEM CELL GENETICS AND GENOMICS

Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation

^aDivision of Hematology and Oncology, Rhode Island Hospital, Providence, Rhode Island, USA;
^bRoger Williams Medical Center, Centers of Biomedical Research Excellence (COBRE), Providence, Rhode Island, USA;
^cDepartment of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island, USA

Key Words. Adult bone marrow stem cells • Bone marrow transplantation • In vitro differentiation • Irradiation • Microvesicles

Correspondence: Jason M. Aliotta, M.D., Division of Hematology and Oncology and Division of Pulmonary, Critical Care and Sleep Medicine, Rhode Island Hospital, 3rd Floor, George Building, 593 Eddy Street, Providence, Rhode Island 02903, USA. Telephone: 401-456-5235; Fax: 401-456-5759; e-mail: jason_aliotta{at}brown.edu

Received February 19, 2007; accepted for publication May 17, 2007.
First published online in STEM CELLS EXPRESS   June 7, 2007.



Numerous animal studies have demonstrated that adult marrow-derived cells can contribute to the cellular component of the lung. Lung injury is a major variable in this process; however, the mechanism remains unknown. We hypothesize that injured lung is capable of inducing epigenetic modifications of marrow cells, influencing them to assume phenotypic characteristics of lung cells. We report that under certain conditions, radiation-injured lung induced expression of pulmonary epithelial cell-specific genes and prosurfactant B protein in cocultured whole bone marrow cells separated by a cell-impermeable membrane. Lung-conditioned media had a similar effect on cocultured whole bone marrow cells and was found to contain pulmonary epithelial cell-specific RNA-filled microvesicles that entered whole bone marrow cells in culture. Also, whole bone marrow cells cocultured with lung had a greater propensity to produce type II pneumocytes after transplantation into irradiated mice. These findings demonstrate alterations of marrow cell phenotype by lung-derived microvesicles and suggest a novel mechanism for marrow cell-directed repair of injured tissue.

Disclosure of potential conflicts of interest is found at the end of this article.

This article has been cited by other articles:

				S. M. Majka, M. Skokan, L. Wheeler, J. Harral, S. Gladson, E. Burnham, J. E. Loyd, K. R. Stenmark, M. Varella-Garcia, and J. West Evidence for cell fusion is absent in vascular lesions associated with pulmonary arterial hypertension Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L1028 - L1039. [Abstract] [Full Text] [PDF]

				J. M. Aliotta, M. Pereira, and P. J. Quesenberry Tissue-Specific Gene Expression of Marrow Cells Co-Cultured with Various Murine Organs Blood (ASH Annual Meeting Abstracts), November 16, 2008; 112(11): 388 - 388. [Abstract]

				J. M Aliotta, N. de Paz, M. Pereira, and P. J. Quesenberry Actinomycin D Blocks Transcription of Surfactant B mRNA in Marrow Cells Co-Cultured with Lung Blood (ASH Annual Meeting Abstracts), November 16, 2008; 112(11): 2898 - 2898. [Abstract]

				D. M. Rodman Proof-of-Concept Trials for Lung Stem Cell Therapy Proceedings of the ATS, August 15, 2008; 5(6): 731 - 735. [Abstract] [Full Text] [PDF]

				D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF]

				D. S. Krause Bone Marrow-derived Cells and Stem Cells in Lung Repair Proceedings of the ATS, April 15, 2008; 5(3): 323 - 327. [Abstract] [Full Text] [PDF]

				J. D. Keene Biological Clocks and the Coordination Theory of RNA Operons and Regulons Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 157 - 165. [Abstract] [PDF]