International Journal of Cell Cloning, Vol 3, 10-21, Copyright © 1985 by AlphaMed Press
Expression of tumor-associated surface membrane antigens on marrow CFU- s, CFC-gm, BFU-e, and brain CFU-s
S Hasthorpe, PF Bartlett and J Rogerson
Antiserum raised against a mouse mast cell line (FMP1) reacts with 90% to
100% of spleen colony-forming units (CFU-s), granulocyte/macrophage
colony-forming cells (CFC-gm), erythroid burst-forming units (BFU-e), and
15% of nucleated marrow cells, using a complement-dependent cytotoxicity
assay. We demonstrated that bone marrow, spleen, or thymus cells are able
to absorb this activity from the antiserum. Although mouse brain cells have
low reactivity with anti-FMP1 serum, the cytolysis level was reduced to
background when antiserum was absorbed with brain cells. In addition,
colony formation by marrow CFU-s, CFC- gm, and BFU-e was no longer
prevented when the cells were incubated with brain-absorbed anti-FMP1 serum
and complement. These findings suggest the presence of brain-associated
antigens on CFU-s, CFC-gm, and BFU-e. To test whether a CFU-s accessory
cell population in marrow is affected by treatment with anti-FMP1 serum and
complement, antibody- treated marrow cells were mixed with large numbers of
thymocytes and injected into recipient mice. Colony formation was not
altered, indicating that the antiserum reacted directly with antigens on
CFU-s and not on CFU-s accessory cells.
