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International Journal of Cell Cloning, Vol 3, 22-32, Copyright © 1985 by AlphaMed Press

ORIGINAL ARTICLES

Enhancement of erythroid progenitor cell growth in medium conditioned by a human cancer cell line, KONT

Y Niho, N Kimura, Y Yamano, Y Yamamoto, Y Hirota, J Ono and R Takaki

Erythroid-potentiating activity (EPA) was detected in culture medium conditioned by a human cancer cell line (KONT) that produces colony- stimulating activity (CSA), using erythroid colony formation in vitro. EPA in the medium conditioned by the KONT cells (KONT-CM) was markedly heat stable. After treating KONT-CM at 80 degrees C for 30 min, 30% EPA remained, while CSA was completely inactivated. Both EPA and CSA appeared in approximately the same fractions of the gel filtration, indicating a molecular weight of approximately 30,000 daltons. EPA bound partially to Concanavalin-A Sepharose, whereas CSA almost did not bind. Our results indicate that EPA can be separated from CSA based on heat stability and binding to Concanavalin-A Sepharose.




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