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International Journal of Cell Cloning, Vol 4, 368-374, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Rapid method for permanent slide preparation of colonies in soft agar cultures

J Moezzi, F Ali-Osman and MJ Murphy Jr

A method is presented for preparing permanent microscopic slides from colony-bearing agar layers in soft agar cultures. The main advantages of this technique are its simplicity, rapidity and accurate colony preservation. This method could have broad applications in the human tumor clonogenic assay (HTCA), particularly in the quantitative morphological, cytochemical and immunocytochemical assessment of colonies that form in both control and drug-treated cultures. Thus, this method opens up possibilities for using cytopathological criteria as a quantitative endpoint of the HTCA.




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Copyright © 1986 by AlphaMed Press.