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International Journal of Cell Cloning, Vol 4, 406-414, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Helper (OKT4) T cells from human T cell colonies produce potent colony- stimulating activity

M Takahashi, H Yamada, MA Bean and JW Singer

Confluent T cell colonies were grown by culturing blood mononuclear cells in double agar layers containing autologous plasma and phytohemagglutinin (PHA) for one week (37 degrees C, 5% CO2). The plates were then overlaid with serum-free alpha medium which was harvested after 24 h. This medium was demonstrated to have colony- stimulating activity (CSA) of greater potency than conventionally prepared PHA-leukocyte conditioned medium, which was prepared by incubating cells from the same donors. Removal of OKT4-positive cells using a monoclonal antibody and complement abolished CSA production by cells from T cell colonies while the removal of OKT8-positive cells had no effect.




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