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International Journal of Cell Cloning, Vol 4, 415-423, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Effect of hydrocortisone and bacterial lipopolysaccharide on colony- stimulating activity production from mouse marrow adherent cells, spleen cells and peritoneal macrophages in vitro

S Aizawa, M Tsurusawa and KJ Mori

The effect of hydrocortisone (HC) on colony-stimulating activity (CSA) production from mouse bone marrow adherent cells, spleen cells and peritoneal macrophages with or without bacterial lipopolysaccharide (LPS) stimulation was studied. CSA in the supernatant from bone marrow adherent cells incubated with HC was found to be five times higher than CSA from cultures without LPS stimulation. In contrast, the CSA production by spleen cells and peritoneal macrophages were significantly suppressed by HC in both LPS-stimulated and non- stimulated cultures. These studies suggest that the effect of HC on CSA production was quite different depending on the target cells.




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