International Journal of Cell Cloning, Vol 5, 302-314, Copyright © 1987 by AlphaMed Press
Production of hemopoietic growth factors and gamma-interferon by large granular lymphocytes from patients with T gamma lymphocytosis
GR Standen, G Masters, R Pill, N Matthews, M Booth and P Baines
Two patients with T gamma lymphocytosis in whom expanded large granular
lymphocyte (LGL) populations were detected in peripheral blood and bone
marrow are described. The surface antigen phenotypes of the LGL from these
patients were similar with a major portion of cells carrying T3, T8, T11
and Leu7 markers. However, whereas fresh LGL from both patients
demonstrated antibody-dependent cell cytotoxicity (ADCC), natural killer
(NK) cell function was present in one case but absent in the other.
Supernatants from enriched suspensions of the LGL unstimulated by exogenous
antigen or mitogens were shown to contain significant amounts of
hemopoietic growth factors colony-stimulating activity (CSA) and
burst-promoting activity (BPA). In one case gamma-interferon was also
detected. This study contributes to the accumulating evidence that LGL are
able to generate factors which have the capacity to influence the
proliferation of hemopoietic progenitor cells in vitro.
