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International Journal of Cell Cloning, Vol 5, 376-384, Copyright © 1987 by AlphaMed Press
ORIGINAL ARTICLES |
YC Dai and JF Prchal
A semisolid culture system for B-cell colony formation is described. The system includes pretreatment of B-cells by neuraminidase-galactose oxidase and help of mitomycin-treated T-cells. With this assay system, colony-forming B-cell precursors were detected in all eight patients we studied with B-cell chronic lymphocytic leukemia. These patients' own T- cell helper effect was less than that of normal T-cells.
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