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International Journal of Cell Cloning, Vol 5, 412-420, Copyright © 1987 by AlphaMed Press

ORIGINAL ARTICLES

Improvement of culture conditions for human megakaryocytic and pluripotent progenitor cells by low oxygen tension

J Katahira and H Mizoguchi

The effect of low oxygen tension on the growth of human hemopoietic progenitor cells in bone marrow was investigated using the semisolid methylcellulose colony assay. The clonal growth of granulocyte- macrophage progenitors (CFU-gm), early (BFU-e) and late (CFU-e) erythroid progenitors, megakaryocyte progenitors (CFU-meg) and pluripotent progenitors (CFU-mix) improved more markedly incubation at the low oxygen tension (5%) than in conventional air (20%). The thiol compound 2-mercaptoethanol had a strong additive effect on colony growth in conventional air, but little or no effect in the low oxygen tension. These results suggest that enhancement of colony growth in the low oxygen tension may be due to a decrease in the production of oxygen intermediates.


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M. A. Dao, M. H. Creer, J. A. Nolta, and C. M. Verfaillie
Biology of umbilical cord blood progenitors in bone marrow niches
Blood, July 1, 2007; 110(1): 74 - 81.
[Abstract] [Full Text] [PDF]


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