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International Journal of Cell Cloning, Vol 5, 480-491, Copyright © 1987 by AlphaMed Press

ORIGINAL ARTICLES

Formation of lymphocyte colonies under serum-free culture conditions in normal individuals and patients with chronic lymphocytic leukemia

YC Dai, GZ Hu, HY Niu, Z Wen, Z Fang, XG Lu, J Li, Q Wu, YP Huang and R Wen
Jiangxi Institute of Medical Science, People's Republic of China.

This paper describes a culture system which supports the formation of B cell and some T cell colonies under serum-free conditions in peripheral blood samples of normal individuals and patients with chronic lymphocytic leukemia (CLL) of B cell type. In this system, serum is replaced by bovine serum albumin, transferrin, cholesterol, insulin and catalase or horseradish peroxidase. In addition, it is necessary to add staphylococcus protein A, mitomycin-treated T cells as feeders and phytohemagglutinin leukocyte-conditioned medium as a source of growth factors. The plating efficiency is greatly enhanced when normal cells are incubated with galactose oxidase prior to plating and when CLL cells are exposed sequentially to neuraminidase and galactose oxidase.




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Copyright © 1987 by AlphaMed Press.