International Journal of Cell Cloning, Vol 5, 504-510, Copyright © 1987 by AlphaMed Press
Synergism of leukemic blast growth factors in medium conditioned by human bladder carcinoma cell line 5637
Y Asano, T Shibuya, S Okamura, E Morioka, S Taniguchi and Y Niho
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Leukemic blast growth factors (LBGFs) are necessary for in vitro growth of
clonogenic cells from patients with acute myeloblastic leukemia. As the
human bladder carcinoma cell line 5637 had previously been reported to
secrete abundant LBGFs into the culture supernatant, the LBGFs in
5637-conditioned medium (5637-CM) were characterized. Measurement of LBGFs
was done using an in vitro leukemic blast colony assay in methylcellulose
culture. LBGFs in 5637-CM were fractionated by anion exchange
chromatography, and two peaks of activity were recovered. Pool B (high-salt
eluent) and/or purified granulocyte colony-stimulating factor (G-CSF) were
added to the clonogenic leukemic blast cell assays. It was found that pool
B was more active than G-CSF in the majority of cases examined and that the
two types of activity were synergistic in some cases.
