International Journal of Cell Cloning, Vol 6, 146-159, Copyright © 1988 by AlphaMed Press
Inhibitory activity of interleukin 2-activated lymphocytes on human granulocyte precursors (CFU-g)
Y Iizuka, T Sakurai, A Horikoshi and T Ohshima
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Interleukin 2-activated lymphocytes (lymphokine-activated killer [LAK]
cells) cultured from 2 to 14 days were added to the cultures of granulocyte
precursors (CFU-g). The LAK cells inhibited colony formation of granulocyte
precursors; LAK cells cultured for five days showed the strongest
inhibitory activity on colony formation. The presence of cell-to-cell
interaction between LAK cells and bone marrow mononuclear cells (BMNC) in
CFU-g assays emphasized the LAK cell- derived colony inhibitory activity
(LAK-CIA), but cell-to-cell interaction was not always a requirement for
LAK-CIA, since LAK cells were also found to inhibit colony formation
without such interaction. This report shows that LAK cells can inhibit in
vitro colony formation of granulocyte precursors. We therefore concluded
that the observed CIA is caused by soluble factor(s) derived from LAK
cells, and that E- rosette-forming cells are manifesting LAK-CIA.
