International Journal of Cell Cloning, Vol 6, 324-340, Copyright © 1988 by AlphaMed Press
A functional analysis of hematopoietic growth factor production from class I and class II human alloreactive T cell clones [published erratum appears in Int J Cell Cloning 1989 Jan;7(1):67]
KF Mangan, A Zeevi, R Duquesnoy, RK Shadduck and AM Gewirtz
Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania.
Class I and Class II human alloreactive T cell clones or their conditioned
media were mixed with progenitor cell-enriched null cells to assess their
ability to stimulate human hematopoietic progenitor cell (HPC) growth.
Optimal release of erythroid, myeloid or megakaryocyte colony-stimulating
factors occurred after 72 hours and required contact of cloned T cells with
irradiated stimulator cells expressing the appropriate major
histocompatibility complex (MHC) determinants recognized by the T cells.
Individual clones were quite heterogeneous in their capacity to release
hematopoietic growth factors. Clones that produced optimal levels of
factors that stimulated granulocyte-macrophage colony growth did not always
produce equivalent amounts of factors that stimulated erythroid colony
growth and vice versa when tested against identical target cells. Class II
clones released nearly twice as much interleukin 3 activity as Class I
clones. Class II clones that lacked cell-mediated lympholytic (CML)
activity against B or T lymphoblastoid targets were consistent stimulators
of HPC growth. In contrast, Class I or Class II clones that contained CML
activity either poorly stimulated or inhibited HPC growth. These CML-
positive clones produced greater amounts of gamma interferon. Our findings
may have important implications for HPC growth following allogeneic
mismatched bone marrow transplantation.
