International Journal of Cell Cloning, Vol 7, 50-58, Copyright © 1989 by AlphaMed Press
Effect of granulocyte-macrophage colony-stimulating factor on chemiluminescence of human neutrophils
S Yamaga, S Okamura, T Otsuka and Y Niho
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
We investigated the capacity of recombinant human granulocyte- macrophage
colony-stimulating factor (rhGM-CSF) to enhance the function of
neutrophils. Neutrophil function was measured in terms of N-formyl-
methionyl-leucyl-phenylalanine (fMLP)-induced luminol-dependent
chemiluminescence (LDCL). LDCL of fMLP-stimulated neutrophils was enhanced
up to 4.5 fold following preincubation with rhGM-CSF. This enhancement
depended on the length of preincubation, reaching an optimal level at 120
min. The dose-response relationship for fMLP- induced LDCL of neutrophils
preincubated with rhGM-CSF revealed that half-maximum enhancement was
achieved at an approximately 20-fold higher concentration than that of
colony-forming units in culture- derived colony formation. These results
suggest that differences in dose dependency may be explained by differences
in the distribution of receptor(s) for GM-CSF. This may also enable GM-CSF
to affect the hematopoietic system, which contains cells at various levels
of differentiation, thus mediating the host-defense mechanism.
