International Journal of Cell Cloning, Vol 7, 190-202, Copyright © 1989 by AlphaMed Press
Effects of inhibitors and activators of protein kinase C on late erythroid progenitor (CFU-e) colony formation in vitro
DM Jenis, CS Johnson and P Furmanski
Laboratory of Cell Biology, AMC Cancer Research Center, Denver, Colorado 80214.
Protein kinase C (PKC) plays a central role in external signal transduction
for many cell types. To examine the involvement of PKC in the control of
erythropoiesis, we tested the effects of PKC inhibitors on in vitro colony
formation by late erythroid progenitors (CFU-e) from normal and Friend
virus-infected mice. Inhibitors of PKC and other kinases (H-7 and H-8)
inhibited CFU-e at concentrations which inhibit PKC. HA1004, an inhibitor
of the cyclic nucleotide-dependent kinases and a weak inhibitor of PKC, had
little effect on CFU-e. In the absence of erythropoietin, a combination of
phorbol ester and Ca++ ionophore significantly increased normal CFU-e.
These results suggest PKC plays a role in the transduction of regulatory
signals for the growth of CFU-e.
