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International Journal of Cell Cloning, Vol 7, 264-280, Copyright © 1989 by AlphaMed Press

REVIEWS

Gene transfer into hemopoietic stem cells using retroviral vectors

JM Chang and GR Johnson
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.

Gene transfer to hemopoietic cells offers a variety of new approaches to the experimental hematologist as well as potentially providing a means for correcting a number of genetic disorders of humans. From the experimental viewpoint, gene transfer utilizing retroviral vectors introduces new methods for analyzing hemopoietic cell lineages, and the effects of over-expression of genes affecting hemopoietic cell proliferation and differentiation. The unique properties of retroviral vectors and the optimized methods currently in use to infect hemopoietic cells are represented as a brief review of a rapidly expanding new field of experimental hematology.




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Copyright © 1989 by AlphaMed Press.