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International Journal of Cell Cloning, Vol 8, 39-53, Copyright © 1990 by AlphaMed Press

ORIGINAL ARTICLES

Specific binding of 125I-rErythropoietin to Friend polycythemia virus- transformed erythroleukemia cells purified by centrifugal elutriation

PN Correa, V Bard and AA Axelrad
Department of Anatomy, University of Toronto, Canada.

We have used countercurrent centrifugal elutriation (CCE) to determine the distribution of cells with respect to cell volume and buoyant density for an erythroleukemia cell line (JG6) transformed by the polycythemia strain of Friend virus (FV-P), and to determine the effect of inducing the cells to differentiate with dimethylsulfoxide (DMSO) on this distribution. CCE made it possible to obtain suspensions of modal JG6 populations virtually free of dead cells and uniform with respect to volume and buoyant density. These modal populations were assayed for specific binding of erythropoietin (Epo). Between 500 and 550 Epo receptors per cell were detected. These belonged to a single class having a dissociation constant of 0.36 nM. DMSO induction of differentiation of the JG6 cells had no effect on the number of Epo receptors expressed.




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