International Journal of Cell Cloning, Vol 9, 233-238, Copyright © 1991 by AlphaMed Press
Carbohydrate composition of human megakaryocyte membranes in culture: identification using binding of seven lectins
JF Abgrall, I Renard, C Berthou, L Sensebe, G Guern, C Autrand, G LeGall and J Briere
Department of Hematology, Augustin Morvan Hospital, Brest University, School of Medicine, France.
Seven tetramethylrhodamine B isothiocyanate- (TRITC) labeled lectins: lens
culinaris (LCH), ulex europeus-1 (UEA-1), lycopersicon esculentum (LEA),
wheat germ agglutinin (WGA), dolichos biflorus (DBA), soybean agglutinin
(SBA) and erythrina cristagalli (ECA) were applied on cultured human
megakaryocytes (Megs) detected by immunofluorescence. All stages of Megs
(from lymphocyte-like Megs to mature Megs) and platelets were labeled by
LCH, LEA, UEA-1 and WGA. ECA binds to platelets but only to some Megs. DBA
did not bind to platelets but did bind to some Megs, irrespective of stage.
SBA binds to all stages of Megs, but did not bind to platelets. These
results indicate the presence of mannose, glucose (LCH), sialic acid (WGA),
and glucosamine (UEA-1, LEA, WGA) on the surface of all cells of the Meg
lineage, a variable presence of galactosamine (DBA, SBA, ECA), and a
discrepancy in the presence of some galactosamine compounds between
platelets and Megs (DBA, SBA).
