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International Journal of Cell Cloning, Vol 9, 559-569, Copyright © 1991 by AlphaMed Press

ORIGINAL ARTICLES

A serum-free medium for the Courtenay-Mills soft agar assay

DP Wilks and CM West
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.

A serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.




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Copyright © 1991 by AlphaMed Press.