Stem Cells http://www.stemcellsportal.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online November 29, 2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2007-0621v1
2007-0621v2
26/3/630    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by May, R.
Right arrow Articles by Houchen, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by May, R.
Right arrow Articles by Houchen, C. W.
Submitted on July 31, 2007
Accepted on November 19, 2007

TISSUE-SPECIFIC STEM CELLS

Identification of a Novel Putative Gastrointestinal Stem Cell and Adenoma Stem Cell Marker: DCAMKL-1 Following Radiation Injury and in APC/Min Mice

Randal May 1, Terrence Riehl 2, Clayton Hunt 3, Sripathi M. Sureban 1, Shrikant Anant 4, Courtney W. Houchen 1*

1 Department of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, OK 73134
2 Department of Internal Medicine, Division of Gastroenterology, Washington University School of Medicine, Saint Louis, MO 63110
3 Department of Radiation Oncology, Radiation and Cancer Biology Division, Washington University School of Medicine, Saint Louis, MO 63110
4 Department of Medicine, Department of Cell Biology, Oklahoma University Health Sciences Center, Oklahoma City, OK 73134

* To whom correspondence should be addressed. E-mail: Courtney-houchen{at}ouhsc.edu.


  Abstract

In the gut, tumorigenesis arises from intestinal or colonic crypt stem cells. Currently no definitive markers exist that reliably identify gut stem cells. Here we utilized the putative stem cell marker doublecortin and CaM kinase-Like (DCAMKL)-1 to examine radiation-induced stem cell apoptosis and adenomatous polyposis coli (APC)/min (multiple intestinal neoplasia) mice to determine the effects of APC mutation on DCAMKL-1 expression. Immunoreactive DCAMKL-1 staining is demonstrated in the intestinal stem cell zone. Furthermore, we observed apoptosis of the cells negative for DCAMKL-1 at 6hr. We found DNA damage in all the cells in the crypt region including the DCAMKL-1 positive cells. We also observed stem cell apoptosis and mitotic DCAMKL-1 expressing cells 24hr after irradiation. Moreover, in APC/min mice, DCAMKL-1 expressing cells were negative for proliferating cell nuclear antigen (PCNA) and nuclear {beta}-catenin in normal appearing intestine. However, {beta}-catenin was nuclear in DCAMKL-1 positive cells in adenomas. Thus nuclear translocation of {beta}-catenin distinguishes normal and adenoma stem cells. Targeting DCAMKL-1 may represent a strategy for developing novel chemotherapeutic agents.

Key Words. Stem Cell Marker, DCAMKL-1, Adenoma Stem Cell Marker, Gamma Radiation, Gastrointestinal Cancer, APC/min mice




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. F. Souza, K. Krishnan, and S. J. Spechler
Acid, Bile, and CDX: the ABCs of making Barrett's metaplasia
Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G211 - G218.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2007 by AlphaMed Press.